Journal: Communications biology

Article Title: FBW7 couples structural integrity with functional output of primary cilia.

doi: 10.1038/s42003-021-02504-4

Figure Lengend Snippet: Fig. 5 FBW7-NDE1 axis affects osteoblast differentiation via effects on primary cilia in C3H10T1/2 cells. a Representative images for ALP staining of wild type, Fbxw7KO, Nde1KO, and Fbxw7Nde1KO C3H10T1/2 cells (n = 3). Images at ×1 or ×2 magnification were taken from 24-well plates. Well diameter: 16 mm. Scale bar: 5 mm. b, c Expression levels of ALP in indicated cells (b) and summary data (c). Data are presented as means ± SEM from four independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test, ****p < 0.0001. d, e mRNA levels of osteoblast differentiation markers Runx2 and Osterix (Osx) in wild type, Fbxw7KO, Nde1KO, and Fbxw7Nde1KO C3H10T1/2 cells. Data are presented as means ± SEM from four or three independent experiments (d or e, respectively). One-way ANOVA with Dunnett’s multiple comparisons test, **p < 0.01, ***p < 0.001. f, g ALP staining after transfection of Fbxw7Nde1KO C3H10T1/2 cells with pcDNA3 or an Ift88-specific sgRNA. Images at ×1 or ×2 magnification were taken from 24-well plates. Well diameter: 16 mm (f). Scale bar: 5 mm. Expression levels of IFT88 in Fbxw7Nde1KO C3H10T1/2 cells transfected with the indicated constructs (g).

Article Snippet: A301‐720A; A301‐721A 1:500), NDEL1 (Abcam, 1:1000), GAPDH (GeneTex, 1:2000), and IFT88 (Proteintech, 1:000).

Techniques: Staining, Expressing, Transfection, Construct